Ppic9 plasmid
WebpPIC9. Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein. Sequence Author: Thermo Fisher ... Home Resources Plasmid Files Yeast Plasmids … Webwas inserted into the pPIC9 K expression vector to form the recombinant plasmid pPIC9 K-FtTI. Expression of the recombinant protein The gene was digested with NotI and EcoRI restriction enzymes, and the generated fragment was ligated into plasmid pPIC9 K. The resulting plasmid (pPIC9 K-FtTI), linearized with SacI restriction enzyme, was used in the
Ppic9 plasmid
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WebMay 3, 2024 · The pPIC9 plasmids carrying the expression cassettes for the two xylanase genes (XynCDBFV (Zhang et al. 2024) and BsXynC (Luo 2008)) were maintained in our lab. For fusion expression of xylanase-SS proteins, the xylanase genes were amplified using gene specific primer pairs (Supplemental Table S1). Webbiochemistry, and plasmid preparation, refer to Molecular Cloning: A Laboratory Manual (Sambrook et al., 1989) or Current Protocols in Molecular Biology (Ausubel et al., 1994). E. …
WebNov 1, 2016 · The fusion gene was cloned into the pPIC9 plasmid to construct the recombinant Pichia pastoris transformant expressing the ompA–Fc fusion protein. The effects of the linked Fc on macrophage vitality, activity, efficiency of antigen processing, and immune responses induced by the fused ompA were investigated. Web86. Buy from Supplier. Pichia Pastoris Expression Vector Ppic9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO …
Web86. Buy from Supplier. Pichia Pastoris Expression Vector Ppic9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. Webvector pPIC9 or pPIC9K using the DNA ligation kit (TAKARA BIO), and the ligation mixture was applied to transform the E. coli DH5 strain. The constructed plasmids (pPIC9/hsTf and pPIC9K/hsTf), purified from the transformants, were confirmed as such using a DNA sequencer. Transformation of P. pastoris. Transformation of P. pastoris was
WebPhysical map of the pPIC-TPP recombinant plasmid showing the insertion of the TPP gene (in blue) into the vector pPIC9, which is flanked by restriction enzymes EcoRI and NotI and …
WebUse XhoI and EcoRI to digest PETase-GCW21/51/61 and pPIC9 at 37 °C. 4. Ligate the digested fragments PETase-GCW21/51/61 along with plasmid pPIC9 digested with XhoI … faculty of arts learning management systemWebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of … dog days of summer bulletin boardWebThe hG-CSF cDNA was cloned into the pPIC9 plasmid under the control of AOX1 promoter. from publication: Production of recombinant human granulocyte-colony stimulating factor by Pichia pastoris ... dog days of summer bosses mlb the show 22WebDec 1, 2003 · The plasmid pPIC9-NotI minus was digested with EcoRI and AvrII, and ligated to the above PCR fragment treated with EcoRI and AvrII to generate plasmid pPIC9-Fc. The ScFv fragment, screened from a phage antibody library (constructed by Qian Feng, unpublished), can bind Glutathione-S-transferase (GST) by PCR amplification using primer … dog days of summer idiom sentencesWebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of … faculty of arts chulalongkorn universityWebJun 9, 2024 · Strains, plasmids, and antibodies. The Pichia pastoris strain GS115, Escherichia coli strain DH5α, and expression vectors pPIC9 and pPICZα were purchased from Invitrogen (Carlsbad, CA). The template plasmids pPIC9-SuIL-15Rα and pPICZα-IgG4-Fc (with mutations S228P, L235E, and N297Q) were constructed and preserved in our … faculty of arts monash universityWebNational Center for Biotechnology Information faculty of arts nottingham