WebNov 8, 2024 · Myosin 1b (Myo1b) is an important single-headed membrane-associated motor of class I myosins that participate in many critical physiological and pathological … WebMyo1b and myosin 1d are expressed in the rodent adult brain, and experi-mental evidence suggests that Myo1b contributes to neurogene-sis (Sherr et al., 1993). Myo1b is adjacent to the plasma membrane of the growth cone in neurons from the superior cervical ganglia (Lewis and Bridgman, 1996). Transcription of myo1b has been
Motor domain-dependent localization of myo1b (myr-1)
WebNov 6, 2015 · To test whether MYO1B was a direct target of miR-363, luciferase reporter assays were performed in JHU028 cells with premiR-363 and reporter plasmids (with wild-type MYO1B 3’ UTR or the MYO1B 3’ UTR with BS1 or BS2 deletion, cloned downstream of the firefly luciferase gene in the pMiR-REPORT™ vector). WebJul 27, 2011 · Myosin I alpha ( MMI-alpha; MMIa) Gene names Name Myo1b Organism names Organism Mus musculus (Mouse) Taxonomic identifier 10090 NCBI Taxonomic … owt company
Myosin 1b promotes cell proliferation, migration, and
WebApr 1, 2015 · Myo1b, a class I myosin, is a widely expressed, single-headed, actin-associated molecular motor, associated with cell migration of zebrafish embryo cells ( 11) and intracellular transport regulated by the … WebNov 15, 2024 · Myo1b promotes cell migration and invasion in vitro and in vivo. A. Western blot analysis for validation of Myo1b knockdown and overexpression in CC cell lines. B. Myo1b knockdown blocked the migration and invasion of CC cells. C. Overexpressed Myo1b enhanced the migration and invasion of C33A cells. The number of cells per field was … We measured the protein surface density (number of proteins per unit area) on solid surfaces or on SLBs by using a previously established procedure36,37. It is calculated from a labeled proteins/lipids calibration. We first measure the fluorescence of POPC SLBs containing predefined amounts of Atto488-DOPE … See more Actin was purified from rabbit muscle and isolated in monomeric form in G buffer (5 mM Tris-HCl, pH 7.8, 0.1 mM CaCl2, 0.2 mM ATP, 1 mM DTT and 0.01% NaN3). Actin was labeled with Alexa 594 succimidyl ester … See more SLBs were formed by fusion of small unilamellar vesicles (SUVs) prepared as follows. Lipid mixtures containing 79.5% POPC, 20% L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) and 0.5% Rhodamine-PE or … See more The kinetics of single filament assembly was monitored by TIRF microscopy (Eclipse Ti inverted microscope, 100× TIRF objectives, Quantem … See more Lipid compositions for GUVs were 79.7% POPC, 20% L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) and 0.3% Texas Red DHPE. GUVs were prepared by using polyvinyl alcohol … See more jeep wrangler with ls engine for sale