Hcclm3-rfp
WebHepatocellular carcinoma (HCC) usually has a dismal prognosis because of its limited response to current pharmacotherapy and high metastatic rate. Sulfated oligosaccharide has been confirmed as having potent antitumor activities against solid tumors. Here, we explo WebNational Center for Biotechnology Information
Hcclm3-rfp
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WebJun 10, 2015 · For orthotopic xenograft model, the HCCLM3-RFP cell line was established by transfecting the HCCLM3 cells with LV-7 RFP plasmid (GenePharma, Shanghai). HCCLM3-RFP cells (1 × 10 7 cells in 0.1 ml ... Web(A) Hierarchical clustering of differentially expressed mRNAs in HCCLM3R, HCCLM3R-LM1-S4 and HCCLM3R-LnM1-S11 cells. (B) Predicted target genes and their involved …
WebJul 16, 2024 · Functional analysis of BMP4 on HCC proliferation and autophagy was performed both in vitro and in vivo in HepG2 and HCCLM3 cells. Autophagic activity was estimated by Western blot for autophagic marker proteins and by transmission electron microscopy (TEM). WebTitle: CCC-853-LPD_Program-Application Author: [email protected] Keywords: CreatedByIRIS_Readiris_16.0.2 Created Date: 5/14/2024 5:19:25 PM
WebNational Center for Biotechnology Information WebLungs from models established with HCCLM3-RFP or HepG2-GFP were excised, and a stereomicroscope imag-ing system (Leica Microsystems Imaging Solutions Ltd) was used to view metastatic foci with fluorescent protein. Metastatic foci exhibiting fluorescence were measured with Image-Pro Plus software (Media Cybernetics, Bethesda,
WebMar 29, 2024 · Background: Angiogenesis is a crucial process in tumorigenesis and development. The role of exosomes derived from hepatocellular carcinoma (HCC) cells in angiogenesis has not been clearly elucidated. Methods and results: Exosomes were isolated from HCC cell lines (HCCLM3, MHCC97L, and PLC/RFP/5) by ultracentrifugation and …
WebXenograft mouse model of HCCLM3-RFP for pulmonary metastasis of hepatocellular carcinoma (HCC). a The flowchart showed the process of creating the xenograft mouse model, HCCLM3-RFP. Stage I,... clifty farms hams for saleWebDec 24, 2024 · GFP-RFP-LC3 dual reporter assay: the three HCC cell lines stably expressing GFP-RFP-LC3 were seeded in the confocal plates and then treated with the … boats for sale in waWebDec 1, 2012 · Ten HCC cell lines (L02, Hep3B, HepG2, SMMC7721, Huh7, MHCC97L, MHCC97H, HCCLM3, HCCLM6 and HCCLM3-RFP) were chosen and cultured in our study. The details were described in supplementary materials and methods. ... HCCLM3-R, a human HCC cell line with high metastatic potential was infected with RFP full-length … clifty farms ham where to buyWebHCCLM3R-LM1-S4 and HCCLM3R-LnM1-S11, 2 monoclonal cell lines, were derived from HCCLM3R-LM1 and HCCLM3R-LnM1 respectively, and exhibited much higher metastatic potentials than HCCLM3-RFP cells. (E) Differentially expressed miRNAs related to HCC metastasis screened by miRNA-seq, aligned according to their chromosome locations. boats for sale in virginia beach vaWeb,q dffrugdqfh zlwk &)5 dssolfdqwv kdyh d frqwlqxlqj reoljdwlrq wr dgylvh wkh &rpplvvlrq wkurxjk dphqgphqwv ri dq\ vxevwdqwldo dqg pdwhuldo fkdqjhv lq wkh lqirupdwlrq … clifty farms pork cracklinsWebDec 1, 2012 · HCCLM3-R, a human HCC cell line with high metastatic potential was infected with RFP full-length cDNA via lentivirus. As shown in Supplementary Fig. 1A, HCCLM3-R expressed red fluorescence, stable and intense. According to our previous study (Wang et al., 2011), none pulmonary metastasis was observed at 1 wk after tumor implantation. boats for sale in wabasha mnWebThis cell line, designated as HCCLM3, was polygonal epithelial cell with hypotriploid karyotype and population doubling time of 34.9 h. The cells were positive for alpha fetoprotein (AFP), albumin, cytokeratin 8 (CK8), and negative for hepatitis B surface antigen (HBsAg) by immunocytochemistry. clifty farm spiral cut ham