http://www.chem-coat.com/ Washing buffer between the steps is Reaction buffer. Apply 100 µl volume of primary and secondary antibodies. 1. Tissue Sample, Paraffin. 2. Deparaffinization in EZ prep 75°C 8 minutes. 3. Cell Conditioning using Conditioner #1, Standard CC1, 95°C 44 minutes. 4. Block with Inhibitor CM, 37°C 4 minutes. 5. No Heat. 6. … See more Paraffin sections of 4 µm thickness are baked overnight at 50°C. The deparaffinization step is done in the Ventana Discovery XT platform using EZ prep solution. See more The standard antigen retrieval method is Heat Induced Epitope Retrieval (HIER) in Tris-EDTA buffer pH 7.8 at 95°C for 44 min (standard CC1) and is performed in the Ventana Discovery XT. If expected results are not … See more For immunohistochemistry, the following reagents are commercially available from Roche, Ventana Medical 1. Systems, Tucson, AZ, USA. 2. EZ Prep Solution, 950-100 3. Reaction … See more For blocking endogenous peroxides and protein, these steps are done in the Ventana Discovery XT platform using Inhibitor CM. … See more
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Web(469) 465-5287 HOME PRODUCTS PHOTOS TRAINING COLOR CHARTS CONTACT. site by sharkmatic WebThe Immunohistochemistry detects antigen in cells or sectioned tissues. It labels antigens of interest by utilizing antibodies' specific binding abilities. motor theory 意味
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WebMar 20, 2024 · To determine oligodendrocyte populations, we stain sections using anti-APC/CC1 antibody and visualize using immunofluorescence. This antibody stains great and makes counting cells easy when sections are … WebDec 27, 2016 · The stroke core is demarcated as the region of loss of oligodendrocytes (CC1 stain) in adjacent sections. ( D) Undifferentiated reporter cells, NG2 + /YFP +, at 7 d after stroke (arrows). ( E) NG2 reporter positive cell stained for a maker of a more mature oligodendrocyte, CC1 (arrow). WebMethyl green has been used in the histological staining of ovaries. [ 1] It has also been used as a counterstain in various cells. [ 2] Packaging 10, 50 g Biochem/physiol Actions … motor theory psychology