Bv2 transfection
WebOct 6, 2024 · BV-2 cells were transfected with either 2R or 66R plasmids using Magnetofection (GL00250; OZ Biosciences) according to manufacturers' instructions. For cell viability assay and to transfect N2a … WebJan 28, 2024 · The BV2 cells received various pretreatments including (a) SRPK2 expression enhancement by transfection of recombinant lentiviral vectors; (b) …
Bv2 transfection
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WebApr 7, 2024 · To simulate the model of RIR in vivo, BV2 cells were cultivated in serum- and glucose-free media and placed under conditions of 5% CO 2 and 95% N 2 in a 37 °C incubator for 3 h, then returned to the normal environment (37 °C, 5% CO 2 and normoxic) and complete medium (DMEM with 10% foetal bovine serum and glucose [4.9 g/L]) for … WebSep 2, 2024 · The BV-2 cell line, developed by Blasi and colleagues [9], is one of the most popular microglial cell lines ever created. It is also one of the oldest; it was established in …
WebApr 9, 2024 · BV2 mouse microglia with the Trem2 H157Y variant were generated using CRISPR-Cas9 technology. ... Trem2 was mutated by co-transfection of plasmids containing the gRNA and Cas9. The transfected BV2 microglia were plated in 96-well plates by limit dilution to generate isogenic single clones. The clones were selected from wells and … WebThe transfection worked well (validated by western blot) and I did not observe cell death. I have also analyzed some gene expression levels and the cells do not seem activated too …
WebFor stably infected cells, BV2 cells were treated with puromycin (2.5 µg/mL, Invitrogen) for 72 hrs. Real-time qPCR and Western blotting assays were used to measure the expression of NF-κB in the BV2 cells. Cell Transfection. BV2 cells (4×10 5 cells per well) were seed onto 6-well plates overnight to reach 70% confluence for transfection ... Web1. Reagents Dulbecco’s Minimum Essential Medium (DMEM) (DMEM is most commonly used, followed by DMEM/F12, MEM/F12). Fetal Bovine Serum (FBS), 10% final concentration. Penicillin/Streptomycin (Pen/Strep), 1 % final concentration (100 IU/ml, 100 μg/ml, respectively). GlutaMAX, 2% final concentration. Trypsin/EDTA. 2.Methods
WebAbstract. Objective: To investigate the role of miR-146a-5p in the effects of resveratrol (RSV) on inflammatory response in BV2 mouse microglial cells. Materials and methods: …
raport mt940 co to jestWebin case you did not solve your problem with siRNA transfection of BV-2 cells yet you can try out Viromer Blue (lipocalyx) and use the reverse transfection protocol. It works perfectly … raport narativ erasmusWebOct 3, 2024 · BV2 cells (4×10 5 cells per well) were seed onto 6-well plates overnight to reach 70% confluence for transfection. Then, miR-340 mimic or miR-34 inhibitor was transfected into cells for 24 hrs using … raport listopad 2020WebJan 1, 2024 · The BV2 cell line is a murine cell line of microglial origin which is often used to study this cell type in vitro. Unfortunately, this microglial cell line is, like other phagocytic … raport listopadWeb此外，meg3 的过表达促进了 m1 极化和炎症，但通过抑制 bv2 细胞中的 klf4 来抑制 m2 极化。 针对 MEG3 的小干扰 RNA 的转染在体外和体内抑制 M1 极化和炎症并促进 M2 极化。 raport najnovijeWebPradeep D Uchil. Liposomal transfection reagents vary in their ability to transfect cell lines efficiently. Some are generalists, whereas others are … drone azerbaijan israelWebjetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids. drone a king jouet